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M94A0356.TXT
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1994-10-08
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Document 0356
DOCN M94A0356
TI TaqStart Antibody: hot start PCR facilitated by a neutralizing
monoclonal antibody directed against Taq DNA polymerase.
DT 9412
AU Kellogg DE; Rybalkin I; Chen S; Mukhamedova N; Vlasik T; Siebert PD;
Chenchik A; CLONTECH Laboratories, Palo Alto, CA.
SO Biotechniques. 1994 Jun;16(6):1134-7. Unique Identifier : AIDSLINE
MED/94355058
AB The specificity and DNA yield of PCRs are often improved by the hot
start technique and analogous methods. The intent of the approach is to
eliminate or prevent the generation of nonspecific PCR templates that
may be synthesized at ambient temperature prior to thermal cycling.
Monoclonal antibodies (MAbs) raised in mice to the purified DNA
polymerase of Thermus aquaticus (Taq) were selected for their ability to
reversibly block polymerase activity. The MAbs, incubated with Taq DNA
polymerase and added to PCR tubes at ambient temperature, yield specific
DNA fragments upon amplification when using high numbers of temperature
cycles and a very low copy number of target DNA in a complex DNA
background. This approach, using the TaqStart Antibody, permits the
preparation of reaction mixtures at ambient temperatures without the
subsequent opening of reaction tubes, use of grease or waxes, or of
degradative enzymes and deoxyribonucleotide analogs.
DE Antibodies, Monoclonal/*PHARMACOLOGY Base Sequence DNA
Polymerases/*ANTAGONISTS & INHIB/IMMUNOLOGY DNA, Viral/ANALYSIS
HIV/GENETICS Molecular Sequence Data Polymerase Chain
Reaction/*METHODS TECHNICAL REPORT JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).